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Durand, M ; Seche, E ; Maurizot, JC

Effect of strand orientation on the interaction of berenil with DNA triple helices

European Biophysics Journal With Biophysics Letters 30 (8) 625-630

par Administrateur - publié le

Abstract :

Using circular dichroism spectroscopy the ability of berenil, a minor groove binding drug, to induce triple helix formation was investigated with two oligonucleotides designed to form two intramolecular triplexes containing T*A:T and G*G:C triplets, which differ only by the orientation of their third strand : 5’-d(G(4)A(4)G(4)-[T-4]-C4T4C4-[T-4]-G(4)T(4)G(4)), and 5’-d(G(4)T(4)G(4)-[T-4]-G(4)A(4)G(4)-[T-4]-C4T4C4), where [T-4] represents a stretch of four thymine residues. We demonstrate that when added to the duplex form of these oligonucleotides, berenil induces triplex structure formation only if the orientation of third strand is anti-parallel to the purine strand.