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de Diesbach, P ; N’Kuli, F ; Berens, C ; Sonveaux, E ; Monsigny, M ; Roche, AC ; Courtoy, PJ

Receptor-mediated endocytosis of phosphodiester oligonucleotides in the HepG2 cell line : evidence for non-conventional intracellular trafficking

Nucleic Acids Research 30 (7) 1512-1521

par Administrateur - publié le , mis à jour le

Abstract :

Having identified an oligonucleotide (ON) receptor in the HepG2 cell line, we have re-examined here the kinetics of ON uptake, subcellular distribution and intracellular localisation in these cells, at concentrations relevant for the study of a receptor-dependent process. Kinetic parameters of ON endocytosis were comparable with those of the receptor-mediated endocytosis tracer, transferrin (uptake equilibrium, saturation with concentration, specific competition and rapid efflux) and were clearly distinct from those of fluid-phase endocytosis.