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Durand, A ; Giardina, T ; Villard, C ; Roussel, A ; Puigserver, A ; Perrier, J

Rat kidney acylase I : further characterisation and mutation studies on the involvement of Glu 147 in the catalytic process

Biochimie 85 (10) 953-962

par Administrateur - publié le

Abstract :

Rat kidney acylase I was characterised by performing site-directed mutagenesis and enzymatic analysis in the presence of various chemical inhibitors. Site-directed mutagenesis on E147 and overexpression of the protein in a bacterial system, revealed the importance of this residue in enzymatic activity, it corresponds to the putative catalytic E 175 in carboxypeptidase G2 from Pseudomonas aeruginosa.