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Spotheim-Maurizot, M ; Begusova, M ; Charlier, M

DNA damaging by ionising radiations. Influence of the structure and of the environment

Actualite Chimique (11-12) 97-102

par Administrateur - publié le

Abstract :

DNA which bears the genetic information of the cell is the critical target for radiolytic attack. In the nucleus, DNA is not a straight and naked double helix in B-form. The sequence of the bases, the topological stress, the interactions with other partners (proteins) induce structures different from the canonical B-form. The effects of radiations occur through the attack by radicals issued from water radiolysis. The most reactive one is the oxidative hydroxyl radical. The radiolytic process is a chemical process in which both reactivity and accessibility of reactive sites, and consequently the structure of DNA, are involved. We have developed a simulation code to calculate the hydroxyl radical attack of different DNA structures. This procedure uses structural data obtained by either NMR, crystallography or molecular modelling, and accurately fits the experimental results.