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Joubert, F ; Mateo, P ; Gillet, B ; Beloeil, JC ; Mazet, JL ; Hoerter, JA

CK flux or direct ATP transfer : Versatility of energy transfer pathways evidenced by NMR in the perfused heart

Molecular and Cellular Biochemistry 256 (1-2) 43-58

par Administrateur - publié le

Abstract :

How the myocardium is able to permanently coordinate its intracellular fluxes of ATP synthesis, transfer and utilization is difficult to investigate in the whole organ due to the cellular complexity. The adult myocardium represents a paradigm of an energetically compartmented cell since 50% of total CK activity is bound in the vicinity of other enzymes ( myofibrillar sarcolemmal and sarcoplasmic reticulum ATPases as well as mitochondrial adenine nucleotide translocator, ANT). Such vicinity of enzymes is well known in vitro as well as in preparations of skinned fibers to influence the kinetic properties of these enzymes and thus the functioning of the subcellular organelles. Intracellular compartmentation has often been neglected in the NMR analysis of CK kinetics in the whole organ. It is indeed a methodological challenge to reveal subcellular kinetics in a working organ by a global approach such as NMR.