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Accueil > Publications > Recherche par années > Années 2000 > 2005

Gillet, B ; Sebrie, C ; Bogaert, A ; Bleneau, S ; de la Porte, S ; Beloeil, JC

Study of muscle regeneration using in vitro 2D H-1 spectroscopy

Biochimica et Biophysica Acta-General Subjects 1724 (3) 333-344

par Administrateur - publié le

Abstract :

The in vivo spectrum of regenerating muscles shows a specific cross-correlation signal assigned to the (n-3) fatty acyl chain, which peaks during the myoblast fusion phase. In order to identify the origin of this signal and to take all the lipid metabolites into account, we investigated the degeneration-regeneration process by H-1 2D NMR of lipid muscle extracts. We observed an increase in the total amount of lipids during the regeneration process, although the lipid profile did not show any drastic change during this process. The changes in the NMR signal observed in vivo and, in particular, the appearance of the specific (n-3) fatty acyl chain signal appears to arise from mobile lipid compartments located in fusing cells. (c) 2005 Published by Elsevier B.V.