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Accueil > Publications > Recherche par années > Années 2000 > 2005

Aci, S ; Mazier, S ; Genest, D

Conformational pathway for the kissing complex - Extended dimer transition of the SL1 stem-loop from genomic HIV-1 RNA as monitored by targeted molecular dynamics techniques

Journal of Molecular Biology 351 (3) 520-530

par Administrateur - publié le , mis à jour le

Abstract :

HIV-1 retroviral genomic RNA dimerization is initiated by loop-loop interactions between the SL1 stem-loops of two identical RNA molecules. The SL1-SL1 unstable resulting kissing complex (KC) then refolds irreversibly into a more stable complex called extended dimer (ED). Although the structures of both types of complex have been determined, very little is known about the conformational pathway corresponding to the transition, owing to the difficulty of observing experimentally intermediate conformations.