Partenaires

CNRS


Rechercher


Accueil > Publications > Recherche par années > Années 2000 > 2005

Mennesson, E ; Erbacher, P ; Piller, V ; Kieda, C ; Midoux, P ; Pichon, C

Transfection efficiency and uptake process of polyplexes in human lung endothetial cells : A comparative study in non-polarized and polarized cells

Journal of Gene Medicine 7 (6) 729-738

par Administrateur - publié le , mis à jour le

Abstract :

Background : Following systemic administration, polyplexes must cross the endothelium barrier to deliver genes to the target cells underneath. To design an efficient gene delivery system into lung epithelium, we evaluated capture and transfection efficiencies of DNA complexed with either Jet-PEI (TM) (PEI-polyplexes) or histidylated polylysine (His-polyplexes) in human lung microvascular endothelial cells (HLMEC) and tracheal epithelial cells.