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Bourgeaux, V ; Piller, F ; Piller, V

Two-step enzymatic synthesis of UDP-N-acetylgalactosamine

Bioorganic & Medicinal Chemistry Letters 15 (24) 5459-5462

par Administrateur - publié le , mis à jour le

Abstract :

UDP-GalNAc has been synthesised with high yield from GalNAc, UTP and ATP using recombinant human GalNAc kinase GK2 and UDP-GalNAc pyrophosphorylase AGX1. Both enzymes have been prepared in one step from I L cultures of transformed Escherichia coli and I he UDP-GalNAc produced has been purified by a simple procedure. The method described is a rapid ;and efficient means to produce UDP-GalNAc as well as analogues like UDP-N-azidoacetylgalactosamine (UDP-GalNAz). (c) 2005 Elsevier Ltd. All rights reserved.