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Gillard, N ; Spotheim-Maurizot, M ; Charlier, M

Radiation abolishes inducer binding to lactose repressor

Radiation Research 163 (4) 433-446

par Administrateur - publié le

Abstract :

The lactose operon functions under the control of the repressor-operator system. Binding of the repressor to the operator prevents the expression of the structural genes. This interaction can be destroyed by the binding of an inducer to the repressor. If ionizing radiations damage the partners, a dramatic dysfunction of the regulation system may be expected. We showed previously that gamma irradiation hinders repressor-operator binding through protein damage. Here we show that irradiation of the repressor abolishes the binding of the gratuitous inducer isopropyl-l-p-D-thiogalactoside (IPTG) to the repressor.