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Accueil > Publications > Recherche par années > Années 2000 > 2006

Chefdor, F ; Benedetti, H ; Depierreux, C ; Delmotte, F ; Morabito, D ; Carpin, S

Osmotic stress sensing in Populus : Components identification of a phosphorelay system

Febs Letters 580 (1) 77-81

par Administrateur - publié le

Abstract :

To study the Populits response to an osmotic stress, we have isolated one cDNA encoding a histidine-aspartate kinase (HK1) and four cDNAs encoding histidine-containing phosphotransfer proteins (HPts), HPt1-4. The predicted HK1 protein shares a typical structure with ATHK1 and SLN1 osmosensors. The 4 HPTs are characterized by the histidine phosphotransfer domain. We have shown that HK1 is upregulated during an osmotic stress in hydroponic culture. We have detected an interaction between HK1 and HPt2, using the yeast two-hybrid system. These results suggest the existence of a multi-step phosphorelay pathway probably involved in osmotic stress sensing in Populus. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.