Accueil > Publications > Recherche par années > Années 2000 > 2006

Smirnov, P ; Lavergne, E ; Gazeau, F ; Lewin, M ; Boissonnas, A ; Doan, BT ; Gillet, B ; Combadiere, C ; Combadiere, B ; Clement, O

In vivo cellular imaging of lymphocyte tracking by MRI : A tumor model approach to cell-based anticancer therapy

Magnetic Resonance in Medicine 56 (3) 498-508

par Administrateur - publié le

Abstract :

The aim of this study was to demonstrate the feasibility of in vivo cell tracking to monitor anticancer cell therapy by means a high-resolution noninvasive MRI method. Ovalbumin-specifi splenocytes (OT-1) labeled with anionic ?-Fe2O3 superparamagnetic iron oxide (SPIO) nanoparticles were adoptively transferred into C57BL/6 mice with growing ovalbumin-expressing tumors. OT-1 cells were tracked in vivo by 7 T MRI 24, 48, an 72 hr after they were injected. The results showed significant negative enhancement of the spleen at 24 hr, and of the tumor at 48 and 72 hr, after labeled cell injection.