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Kocanova, S ; Hornakova, T ; Hritz, J ; Jancura, D ; Chorvat, D ; Mateasik, A ; Ulicny, J ; Refregiers, M ; Maurizot, JC ; Miskovsky, P

Characterization of the interaction of hypericin with protein kinase C in U-87 MG human glioma cells

Photochemistry and Photobiology 82 (3) 720-728

par Administrateur - publié le

Abstract :

A fluorescence imaging technique was used to monitor intracellular localization of protein kinase C (PKC) in U-87 MG human glioma cells in the presence of hypericin (Hyp) and phorbol 12-myristate-13-acetate (PMA). It is shown that PKC localization, which reflects its activity, is influenced by Hyp and this influence is different from that observed for PMA which acts as PKC activator. Fluorescence binding experiments were used to determine the binding constants of Hyp to several isoforms of PKC. The obtained values of K(d)s (similar to 100 nM) suggest that Hyp binds with high affinity to PKC.