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Accueil > Publications > Recherche par années > Années 2000 > 2007

Chassignol, M ; Aubert, Y ; Roig, V ; Asseline, U

Detection of terminal mismatches on DNA duplexes in homogeneous assays or with immobilized probes

Nucleosides Nucleotides & Nucleic Acids 26 1669-1672

par Administrateur - publié le

Abstract :

We recently reported the design of new fluorescent oligo-2’-deoxyribonucleotides (FODNs) for the detection of terminal mismatches on DNA duplexes in homogeneous assays. We now report the validation of this method in homogeneous assays with other sequences and the feasibility of the detection of terminal mismatches with immobilized FODNs. In all cases studied, the mismatched duplexes were more fluorescent than the perfect ones and results confirmed that the discrimination factor is sequence-dependent.