Accueil > Publications > Recherche par années > Années 2000 > 2008

Bure, C ; Goffinont, S ; Delmas, AF ; Cadene, M ; Culard, F

Oxidation-sensitive residues mediate the DNA bending abilities of the architectural MC1 protein

Journal of Molecular Biology 376 (1) 120-130

par Administrateur - publié le , mis à jour le

Abstract :

The Methanosarcina thermophila MC1 protein is a small basic protein that is able to bend DNA sharply. When this protein is submitted to oxidative stress through gamma irradiation, it loses its original DNA interaction properties. The protein can still bind DNA but its ability to bend DNA is decreased dramatically. Here, we used different approaches to determine the oxidations that are responsible for this inactivation. Through a combination of proteolysis and mass spectrometry we have identified the three residues that are oxidized preferentially.