Accueil > Publications > Recherche par années > Années 1990 > 1997

Asseline, U ; Thuong, NT ; Helene, C

Synthesis and properties of oligonucleotides covalently linked to intercalating agents

New Journal of Chemistry 21 (1) 5-17

par Administrateur - publié le

Abstract :

The chemical precesses involved in the preparation of oligonucleotide-intercalator conjugates are reviewed. Two strategies are used to covalently attach an intercalating agent to oligonucleotides ; it can be either directly incorporated during the oligonucleotide chain assembly or linked to the unblocked oligonucleotide using a specific coupling reaction. The covalent attachment of different intercalating agents to various positions of an oligonucleotide chain : 5’-, 3’-, both 5’- and 3’-ends, internucleotidic phosphate, 1’- or 2’-position of a sugar residue or nucleic base is reported, Studies of the binding of these modified oligonucleotides to their targets are also presented together with some of the unique properties which are conferred upon the oligonuleotides by attachment of an intercalating agent.