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Boudvillain, M ; Guerin, M ; Dalbies, R ; SaisonBehmoaras, T ; Leng, M

Transplatin-modified oligo(2’-O-methyl ribonucleotide)s : A new tool for selective modulation of gene expression

Biochemistry 36 (10) 2925-2931

par Administrateur - publié le , mis à jour le

Abstract :

In the reaction between trans-diamminedichloroplatinum(II) and single-stranded oligo(2’-O-methyl ribonucleotide)s containing the sequence GNG (N being a nucleotide residue), the 1,3-trans-(Pt (NH3)(2)[GNG]} cross-links are formed. The 1,3-intrastrand cross-links are inert within the single-stranded oligonucleotides. By contrast, they rearrange into interstrand cross-links when the platinated oligonucleotides are paired with their complementary RNA strands. The rate of the interstrand cross-linking reaction depends upon the sequence facing the intrastrand cross-links.