Accueil > Publications > Recherche par années > Années 1990 > 1997

Bourgerie, SJ ; Michan, CM ; Thomas, MS ; Busby, SJW ; Hyde, EI

DNA binding and DNA bending by the MelR transcription activator protein from Escherichia coli

Nucleic Acids Research 25 (9) 1685-1693

par Administrateur - publié le

Abstract :

The Escherichia coli melR gene encodes MelR protein which is a member of the AraC/XylS family of bacterial transcription activators, The function of MelR was investigated by making a targeted deletion in the melR gene of the Escherichia coli chromosome, MelR is a transcription activator essential for melibiose-dependent expression of the melAB operon which is needed for bacterial growth with melibiose as a carbon source. To investigate the interactions of MelR at the melAB promoter, both full length MelR and a shortened derivative, MelR173, containing the C-terminal DNA-binding domain, were purified as fusions to glutathione-S-transferase. Circular permutation studies show that both full-length MelR and MelR173 induce an apparent bend upon binding to target sites at the melAB promoter, Bound full-length MelR, but not MelR173, can oligomerise to form larger complexes that are likely to be involved in transcription activation.