Valverde, I., Lecaille, F., Lalmanach, G., Aucagne, V. & Delmas., A.F.
Synthesis of a biologically active triazole-containing analogue of cystatin A through successive peptidomimetic alkyne–azide ligations
Angew. Chem. Int. Ed. 2012, 51 (10) 718-722
“Click” protein : CuI-catalyzed cycloaddition of azides and terminal alkynes has been applied to the successive ligations of three unprotected peptide fragments. Peptidomimetic triazole ligation (PTL, see scheme) as a new method for the chemical production of bioactive proteins is applied for the synthesis of a triazole-containing analogue of the 97 amino acid protein cystatin A.