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Botte, C. Y. ; Deligny, M. ; Roccia, A. ; Bonneau, A. L. ; Saidani, N. ; Hardre, H. ; Aci, S. ; Yamaryo-Botte, Y. ; Jouhet, J. ; Dubots, E. ; Loizeau, K. ; Bastien, O. ; Brehelin, L. ; Joyard, J. ; Cintrat, J. C. ; Falconet, D. ; Block, M. A. ; Rousseau, B. ; Lopez, R. and Marechal, E.,

Chemical inhibitors of monogalactosyldiacylglycerol synthases in Arabidopsis thaliana

Nat. Chem. Biol. 7 (11) 834-842

par Frapart - publié le

Abstract :

Monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) are the main lipids in photosynthetic membranes in plant cells. They are synthesized in the envelope surrounding plastids by MGD and DGD galactosyltransferases. These galacto-lipids are critical for the biogenesis of photosynthetic membranes, and they act as a source of polyunsaturated fatty acids for the whole cell and as phospholipid surrogates in phosphate shortage. Based on a high-throughput chemical screen, we have characterized a new compound, galvestine-1, that inhibits MGDs in vitro by competing with diacylglycerol binding. Consistent effects of galvestine-1 on Arabidopsis thaliana include root uptake, circulation in the xylem and mesophyll, inhibition of MGDs in vivo causing a reduction of MGDG content and impairment of chloroplast development. The effects on pollen germination shed light on the contribution of galactolipids to pollen-tube elongation. The whole-genome transcriptional response of Arabidopsis points to the potential benefits of galvestine-1 as a unique tool to study lipid homeostasis in plants.