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Accueil > Publications > Recherche par années > Années 2010 > 2012

Aucagne, V., Valverde, I.E., Marceau, P., Galibert, M., Dendane, N., Delmas, A.F.

Towards the Simplification of Protein Synthesis : Iterative Solid-Supported Ligations with Concomitant Purifications

Angewandte Chemie International Edition, 51 (45) 11320-11324

par Frapart - publié le , mis à jour le

Abstract :

Please release me : A new linker for the temporary tagging of peptides at their N-terminus after solid-phase elongation, and its potential for capture/release purification is demonstrated. This concept is extended to a remarkably efficient self-purifying N-to-C iterative triazole ligation strategy, which is applied to the synthesis of a polypeptide having 160 residues, in a high purity without the need for chromatographic purification (see picture ; orange blocks : peptide segments).