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Brevet, D., Hocine, O., Delalande, A., Raehm, L., Charnay, C., Midoux, P., Durand, J.O. and Pichon, C.

Improved gene transfer with histidine-functionalized mesoporous silica nanoparticles

International Journal of Pharmaceutics (2014) 471 (1-2) 197-205 - doi : 10.1016/j.ijpharm.2014.05.020

by Frapart - published on , updated on


Mesoporous silica nanoparticles (MSN) were functionalized with aminopropyltriethoxysilane (MSN-NH2) then l-histidine (MSN-His) for pDNA delivery in cells and in vivo. The complexation of pDNA with MSN-NH2 and MSN-His was first studied with gel shift assay. pDNA complexed with MSN-His was better protected from DNase degradation than with MSN-NH2. An improvement of the transfection efficiency in cells was observed with MSN-His/pDNA compared to MSN-NH2/pDNA, which could be explained by a better internalization of MSN-His. The improvement of the transfection efficiency with MSN-His was also observed for gene transfer in Achilles tendons in vivo.