Sarou-Kanian V., Joudiou N., Louat F., Yon M., Szeremeta F., Meme S., Massiot D., Decoville M., Fayon F., Beloeil J.-C.
Scientific Reports (2015 5 9872 - doi : 10.1038/srep09872
publié le , mis à jour le
We have developed new methods enabling in vivo localization and identification of metabolites through their (1)H NMR signatures, in a drosophila. Metabolic profiles in localized regions were obtained using HR-MAS Slice Localized Spectroscopy and Chemical Shift Imaging at high magnetic fields. These methods enabled measurement of metabolite contents in anatomic regions of the fly, demonstrated by a decrease in beta-alanine signals in the thorax of flies showing muscle degeneration.