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Pansieri, J., Plissonneau, M., Stransky-Heilkron, N., Dumoulin, M., Heinrich-Balard, L., Rivory, P., Morfin, J.-F., Eva, T., Maria Joao, S., Allémann, E., Tillement, O., Forge, V., Lux, F. and Marquette, C.

Multimodal imaging Gd-nanoparticles functionalized with Pittsburgh compound B or a nanobody for amyloid plaques targeting

Nanomedicine (2017) 12 (14) 1675-1687

par Frapart - publié le

Abstract :

Aim : Gadolinium-based nanoparticles were functionalized with either the Pittsburgh compound B or a nanobody (B10AP) in order to create multimodal tools for an early diagnosis of amyloidoses.
Materials & methods : The ability of the functionalized nanoparticles to target amyloid fibrils made of β-amyloid peptide, amylin or Val30Met-mutated transthyretin formed in vitro or from pathological tissues was investigated by a range of spectroscopic and biophysics techniques including fluorescence microscopy. Results : Nanoparticles functionalized by both probes efficiently interacted with the three types of amyloid fibrils, with KD values in 10 micromolar and 10 nanomolar range for, respectively, Pittsburgh compound B and B10AP nanoparticles. Moreover, they allowed the detection of amyloid deposits on pathological tissues.
Conclusion : Such functionalized nanoparticles could represent promising flexible and multimodal imaging tools for the early diagnostic of amyloid diseases, in other words, Alzheimer’s disease, Type 2 diabetes mellitus and the familial amyloidotic polyneuropathy.

Voir en ligne : 10.2217/nnm-2017-0079