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Optical spectroscopy facility

This facility consists of several instruments based on optical spectroscopy for the biophysical characterization of biological samples.
The optical spectroscopy platform is primarily available for CBM investigators, but remains open to all members of the scientific community - both academic and privately-funded.

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Our facility is primarily a user-based facility where researchers are trained on the care and handling of the equipment. In addition to providing training for new users, our staff is available to help users in the design of experiments and the interpretation of results. It is also possible to agree that experiments are fully performed by the staff, for feasibility testing, for example.

The facility provides access to instruments for the following measurements:
-  UV-visible absorption spectrophotometry
-  Circular dichroism
-  Microplates readings
-  Surface plasmon resonance

UV-visible absorption spectrophotometry

UV-Vis spectroscopy probes the electronic transitions of molecules that absorb light in the UV and visible regions of the electromagnetic spectrum.
This can be used to identify substances in a sample, to determinate concentration of proteins or nucleic acids, to study enzymatic assays, etc.
Two spectrophotometers are available at the facility, both standard research-grade UV/Vis double-beam spectrophotometer. The V670 system provides users with excellent performances: extended spectral range (190 - 2700nm), very low noise level (0.0005 OD), extended linearity (>4 OD in the visible, >3 OD in the NIR).

Available instrumentations: Uvikon XL (Secomam); V670 (Jasco) with automatic 6-position Peltier cell changer and integrating sphere

Uvikon XL

Jasco V670

Circular Dichroism

Circular dichroism is an excellent method for analyzing protein and nucleic acid secondary structure in solution. It can also be used to follow the changes in folding as a function of temperature (or pH, buffer …), or for measuring protein-ligand and nucleic acid-ligand interactions.
The Jasco J-810 spectropolarimeter is research-grade system, spectral measurement can be recorded down to 180 nm.

Available instrumentation: J810 (Jasco) with Peltier temperature control and stopped-flow.

Jasco J810

Microplate reading

Two systems are available, one multilabel system and one more specifically dedicated to luminescence studies.
The Victor microplate reader is suitable for 5 types of measurements: Photometry, Luminescence, Fluorescence, Polarized Fluorescence and Time resolved Fluorescence.
The Mithras is dedicated to luminescence but can also perform fluorescence and absorbance readings. It is equipped with two injectors.
A variety of filters (excitation and emission) is available for both systems.

Available instrumentations: Victor 3V (Perkin Elmer), Mithras (Berthold)

Victor 3V


Surface Plasmon Resonance

Biacore is a biosensor technology for monitoring molecular interactions in real time, without labelling. Molecules can include peptides, proteins, nucleic acids, carbohydrates Interactions are detected using the optical phenomenon surface plasmon resonance, which detects changes in the refractive index of the solution close to the sensor chip surface. Briefly, receptor molecules are immobilized on the surface of a sensor chip, then a solution containing potential binding partners is passed over the modified surface. Any change in surface concentration resulting from an interaction is detected and displayed as a function of time, providing a real-time record of molecular associations and dissociations.

Available instrumentation: Biacore 3000 (GE Healthcare)

Biacore 3000

VILLETTE Sandrine CNRS research engineer,
Technical manager Tel : 02 38 25 55 38

HAMACEK Josef Professor, University of Orleans, Scientific manager Tel : 02 38 25 55 82

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