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Accueil > Publications > Recherche par années > Années 1980 > 1983

1983

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Micro carrier culture of fibroblastic cells on modified tris acryl beads

New microcarriers (Trisacryl beads) based on the copolymerization of 2-acrylamido-2-hydroxymethylpropane-1,3-diol and N,N’-diallyltartradiamide were synthesized in order to grow cells requiring attachment to a solid matrix. These microcarriers have a very low content of ionic charges, their density is .apprx. 1.05 and they are only permeable to small molecules ; they are devoid of cytotoxicity. Their hydrophilicity prevents binding or adsorption of cells onto their surface. Gelatin-coupled Trisacryl beads and heparin-substituted Trisacryl beads were prepared. These gelatin or heparin Trisacryl beads are sterilized by autoclaving. BHK 21 C 13 cells readily attach to and spread on gelatin-coupled Trisacryl beads and on fibronectin-precoated heparin-substituted Trisacryl beads. The growth rate of BHK 21 C 13 cells attached on gelatin-coupled Triascryl beads is as high as the growth rate of cells attached to plastic culture dishes (doubling time of 14 h). Cells attached to gelatin-coupled Trisacryl beads are detached by a trypsin-disodium EDTA treatment. When beads covered with BHK 21 C 13 cells were inoculated in culture dishes containing cell-free gelatin-coupled Trisacryl beads, cells spontaneously colonized the cell-free beads.

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