Identification, occurrence and prevention of aspartimide-related byproducts in chemical protein synthesis
Published on July 28, 2025Aspartimide formation is an overlooked side-reaction occurring during chemical ligation-based protein synthesis. We developed a straightforward and potentially generally applicable methodology to prevent it, based on a backbone nitrogen protection.
Chem. Sci., 2025, Advance Article
https://doi.org/10.1039/D5SC03824