PhD thesis of Ayyoub Rayyad (Quantification of clinical mAb solutions using Raman spectroscopy: Macroscopic vs microscopic analysis)

Summary

Raman Spectroscopy is well emerged in the field of Analytical Quality Control (AQC) as a rapid and cost-effective technique useful in many applications. In this study, the potential of Raman spectroscopy was investigated for analysis of clinical preparations of mAbs. Three commercial formulations of monoclonal antibodies (mAbs) Avastin®, Ontruzant® and Tecentriq® corresponding to Bevacizumab (BVC), Trastuzumab (TRS) and Atezolizumab (ATZ) respectively, were analysed in quartz cuvette (macroscopic mode) and through the wall of perfusion bags (microscopic mode). For quantitative analysis, models have been constructed using Partial Least Square Regression (PLSR) with Leave K-Out Cross Validation (LKOCV). The quantification performance was comparable for both macroscopic and microscopic modes, in terms of error and linearity. The results are thus promising for future AQC in situ, in perfusion bags.

Paper : A. Rayyad et al., Talanta, vol. 250, p. 123692, 2022, doi: 10.1016/j.talanta.2022.123692.