Instrumentation

Spectroscopy

Raman Spectrophotometers

	Confocal Raman/fluorescence microspectrometer LabRAM (Jobin-Yvon Horiba) 491 nm 633 nm 691 nm 785 nm micromanipulated femto-injector thermostated micro-chamber
	Confocal Raman/fluorescence microspectrometer LabRam Soleil (Jobin-Yvon Horiba) 473 nm 532 nm 638 nm 691 nm 2 detectors dark field lighting
	Confocal Raman/fluorescence microspectrometer WiTec available excitation wavelengths: 532 nm 633 nm 785 nm
	Raman skin composition analyser RiverD gen2-SCA measurement window for in-vivo skin analysis 785 nm 830 nm
	Industrial multichannel Raman monitor Viserion (Indatech) for online monitoring of bioreactors 4 fiber optic Raman probes for simultaneous measurements 785 nm (3 independent channels) 830 nm (1 channel)

IR Spectrophotometer

infrared spectrometer - Perkin Elmer Frontier FTIR

FTIR spectrophotometer
Frontier (Perkin Elmer)

ATR accessory

Fluorescence Spectrophotometers

fluorescence spectrophotometer - Edinburgh Instruments FS5

Fluorescence spectrophotometer
FS5 (Edinburgh Instruments)

thermostated cell
integration sphere available

spectrofluorimeter - Agilent Cary Eclipse

Fluorescence spectrophotometer
Cary Eclipse (Agilent)

fiber optics accessory available

Separation techniques

capillary electrophoresis - Beckman P/ACE MDQ

Capillary electrophoresis
P/ACE MDQ (Beckman Coulter)

diode array detector
laser induced fluorescence (488 nm)

HPLC Vanquish (Thermo), Nexera (Shimadzu)

Liquid Chromatography U-HPLC
Vanquish (Thermo Fisher Scientific)
Nexera (Shimadzu)

high pressure gradient
diode array detector
fluorescence detector
biocompatible

Liquid Chromatography U-HPLC
1290 Infinity II Bio (Agilent)

diode array detector
high pressure gradient
biocompatible

Physical characterization

particle sizing, zeta potential, rheology, magnetic heating capacity

dynamic light scattering - Malvern Zetasizer Advance PRO

Dynamic light scattering (DLS) and zeta potential
Zetasizer Advance PRO (Malvern)

Rheometer Kinexus pro+ (Netzsch)

type: rotational
type of samples: fluids, semi-solids
measures: viscosity, rheology, viscoelasticity
torque range: 1.0 - 225 nN.m
temperature range: 0 - 150 °C

Calorimetry for magnetic nanoparticles
MagneTherm (nanoTherics)
allows to study the impact of an alternative magnetic field on the heating capacity of nanovectors (NV) in real time

Options:

Drug Release: study of the release of drugs that are loaded on the NV in suspension
In vitro: study of the NV on cells
In vivo: study of the NV on small animals (mice)

Biochemical characterization

Photometric analyzer
Cedex Bio (Roche)

reagent kits available :

glucose, glutamate, glutamine, lactate, LDH, IgG, total protein, ammonium, sodium, potassium

Biolayer interferometer Octet RED96e
(FortéBio)

Diapo imaging system Fusion Solo S - Vilber Lourmat

Imaging system Fusion Solo S
(Vilber Lourmat)
for Western blot, DNA and RNA gels
(chemiluminescence, UV absorbance

Cell culture and biological characterization

	Bioreactor for culture of animal cells and controller Neptune (Pierre Guérin) total volume 4.6 L working volume 2.2 - 3.0 L pH, oxygen and optical density probes adapters for 2 Raman optical fiber probes adapter for a UV-vis probe
	Microbiological safety cabinets, CO₂ incubators for pre-culture
	Cell density counter NucleoCounter (Chemometec) viable and total cell densities
	Cryostat for tissue sectioning CM3050 S (Leica) type of samples : skin (human, reconstructed, synthetic) other tissues cultured cells temperature range: -40 - 0 °C section thickness: 0.5 - 300 µm dimensions of samples: 40 x 55 mm

Formulation

Ultrasound processor UP100H (Hielscher)
100 W, 30 kHz
volume 5 - 250 mL

emulsification
dispersion
homogenization
disagglomeration of nanomaterials
degassing

Disperser ULTRA-TURRAX T 10 - IKA
speed 8000 - 30 000 rpm
volume 1 - 100 mL

emulsification
dispersion
homogenization