METABOLOMICS

The aim of metabolomic studies is to identify the metabolic pathways affected by diseases or treatments in order to improve our understanding of the molecular phenomena involved and enable the identification of new therapeutic targets or even biomarkers.

We are mainly developing NMR metabolomic analysis on Drosophila models of human diseases cancers (https://doi.org/10.1021/acs.jproteome.1c00304, https://doi.org/10.1096/fj.202300783RR) and neurodegenerative diseases such as Huntington's disease (https://dx.doi.org/10.1021/acs.jproteome.0c00335) or Parkinson's disease (in prep). Other analyses are also being carried out on cellular models, for example to study the effects of wood knot extracts on skin cells (https://doi.org/10.1021/acs.jproteome.4c00836)

Left: Graphical summary of a metabolomics study on the example of Huntington's disease (https://dx.doi.org/10.1021/acs.jproteome.0c00335): i) Construction of the Drosophila model carrying Huntington's disease, ii) NMR analyses, iii) Statistical analyses and iv) Identification of disease biomarkers. Right: 13C-HSQC NMR spectra of extracts from control (blue) and glioma (red) Drosophila, fed 13C glucose. Signals from lipids (clearly visible on enlargements) are considerably reduced in flies carrying gliomas, while lactate is increased (https://doi.org/10.1096/fj.202300783RR ).