Accueil > Publications > Recherche par années > Années 1990 > 1991

Grillon, C ; Monsigny, M ; Kieda, C

Soluble human lymphocyte sugar binding-proteins with immunosuppressive activity

Immunology Letters 28 (1) 47-56

par Administrateur - publié le

Abstract :

Suppression of the immune response, which involves suppressor factors released from specialized T cells, is inhibited by a-L-rhamnose. In this paper, we show the presence of rhamnose-specific receptors on a human CD8+ T cell-rich population and describe a novel method to isolate cells which express a given sugar-binding protein on their surface. We describe the isolation of a-L-rhamnose-specific molecules (rhamnose-binding fractions : RBF) from a water-soluble extract from lymphocytes, their purification by affinity chromatography on immobilized neoglycoproteins containing rhamnose residues. RBF kept their ability to bind rhamnose, as shown by the binding of fluorescein-labeled RBF to rhamnosylated BSA-substituted beads. RBF efficiently suppresses DNA synthesis of mitogen-stimulated human lymphocytes as well as B cell immunoglobulin production. Therefore, these rhamnose-binding molecules appear to be antigen-independent suppressor factors.