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Legrand, A ; Greenspan, P ; Nagpal, ML ; Nachtigal, SA ; Nachtigal, M

Characterization of human vascular smooth muscle cells transformed by the early genetic region of SV40 virus

American Journal of Pathology 139 (3) 629-640

par Administrateur - publié le

Abstract :

Human arterial smooth muscle cells transfected with the plasmid pSV3-neo, which contains the SV40 virus early region and the neo(r) gene, developed colonies of morphologically transformed cells. Five cell strains were initiated from these colonies and could be subcultivated for up to 9 months before entering a stage of crisis that ended their life span. Deoxyribonucleic acid (DNA) molecules containing viral sequences were found free and integrated in the transformed cells. The intranuclear SV40 large T antigen and the p53 cellular protein were expressed in the transformed cells.