Accueil > Publications > Recherche par années > Années 1990 > 1993

Gell, V ; Lloubes, R ; Zaat, SAJ ; Vanspaendonk, RML ; Rollin, C ; Benedetti, H ; Lazdunski, C

Recognition of the colicin-a n-terminal epitope 1c11 in vitro and in vivo in escherichia-coli by its cognate monoclonal-antibody

Fems Microbiology Letters 109 (2-3) 335-342

par Administrateur - publié le

Abstract :

We demonstrate that the 1C10 monoclonal antibody (mAb) directed against the N-terminal domain of the colicin A recognizes a 13 residue-region (13 Thr-Gly-Trp-Ser-Ser-Glu-Arg-Gly-Ser-Gly-Pro-Asp-Pro25). When this peptide is inserted into a protein in the amino-terminal or an internal position, the tagged protein is efficiently detected by the 1C11 mAb either by immunoblotting or immunoprecipitation. In vitro, the minimal structure required for detection using the pepscan system is 19Arg-Gly-Ser-Gly-Pro-Glu-Pro25, indicating that in vivo the proper exposure of the epitope requires additional residues. The construction of a versatile vector allowing overproduction of tagged proteins is described. Various applications of the 1C11 epitope are mentioned. This epitope did not alter the function of any of the proteins so far tested.