Accueil > Publications > Recherche par années > Années 1990 > 1993

Amin, AR ; Tamma, SML ; Swenson, CD ; Kieda, CC ; Oppenheim, JD ; Finkelman, FD ; Coico, RF

The immunoaugmenting properties of murine IGD reside in its C(d)1 and C(d)3 regions - potential role for IGD-associated glycans

International Immunology 5 (6) 607-614

par Administrateur - publié le

Abstract :

IgD receptor (IgD-R) bearing CD4+ T cells with immunoaugmenting properties in vivo are induced in mice within 24 h after a single injection of dimeric or aggregated IgD. In the present study, we sought to identify the region(s) of IgD responsible for upregulation of IgD-R and for the immunoaugmenting effect of IgD. IgD-R can be upregulated on CD4+ T cells in vitro and in vivo by glutaraldehyde-aggregated mutant IgD or by fragments of enzymatically digested IgD molecules possessing either the C(d)1 domain (Fd(d)) or the C(d)3 domain (Fc(d)). Neoglycoproteins (D-galactose - BSA and N-acetyl-D-glucosamine - BSA), can competitively block upregulation of IgD-R by IgD in vitro. Furthermore, when injected 1 day before antigen, the aggregated IgD derived molecules, KWD1 (which lacks C(d)1), KWD6 (which lacks C(d)1 plus C(d)-hinge), and Fab(d) can all cause augmentation of antigen-specific primary and secondary antibody responses comparable to that achieved with intact aggregated IgD.