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Godard, G ; Francois, JC ; Duroux, I ; Asseline, U ; Chassignol, M ; Thuong, NT ; Helene, C ; Saisonbehmoaras, T

Photochemically and chemically activatable antisense oligonucleotides - comparison of their reactivities towards DNA and RNA targets

Nucleic Acids Research 22 (22) 4789-4795

par Administrateur - publié le

Abstract :

Dodecadeoxyribonucleotides derivatized with 1,10-phenanthroline or psoralen were targeted to the point mutation (G double right arrow U) in codon 12 of the Ha-ras mRNA. DNA and RNA fragments, 27 nucleotides in length, and containing the complementary sequence of the 12mers, were used to compare tire reactivity of the activatable dodecamers (cleavage oil the target by the phenanthroline-12mer conjugates ; photo-induced cross-linking of psoralen-12mer conjugates to the target). The reactivity of the RNA with the dodecamers was weaker than that of the DNA target. With psoralen-substituted oligonucleotides, it was possible to obtain complete discrimination between the mutated target (which contained a psoralen-reactive T(U) in the 12th codon) and the normal target (which contained G at the same position).