Accueil > Publications > Recherche par années > Années 1990 > 1994

Cizeau, J ; Decoville, M ; Leng, M ; Locker, D

Deletions induced in the white and vermilion genes of drosophila-melanogaster by the antitumor drug cis-dichlorodiammineplatinum(ii)

Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis 311 (1) 31-38

par Administrateur - publié le

Abstract :

This paper describes the analysis of cisplatin induced mutations at the white (w) and vermilion (upsilon) loci located on the X chromosome of Drosophila melanogaster. Twenty-eight w and eight upsilon mutants have been found in a male genetic context and 42 w mutants in a female genetic context. In these latter experiments, genetic analysis showed the presence of multi-locus deficiencies in 18 out of 42 w mutants. Eighteen w and three upsilon intragenic mutations were analyzed at the molecular level. Seventeen w and three upsilon mutants carry deletions within the gene, ranging in size from 4 to 109 base pairs. Sequence analysis of the mutants indicates that most of them were produced by non-homologous recombinational events occurring between short (2-5 bp) sequence repeats on both sides of the deletion, one repeat being retained at the new junction. These results differ largely from those obtained in prokaryotic and other eukaryotic cells.