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Fargeas, C ; Hommel, M ; Maingon, R ; Dourado, C ; Monsigny, M ; Mayer, R

Synthetic peptide-based enzyme-linked immunosorbent assay for serodiagnosis of visceral leishmaniasis

Journal of Clinical Microbiology 34 (2) 241-248

par Administrateur - publié le

Abstract :

Synthetic peptides, derived from the amino acid sequence of a Leishmania donovani clone, were used to develop an enzyme-linked immunosorbent assay (ELISA) for detecting antibodies against L. donovani. For this purpose, five peptides were conjugated to a protein carrier, human serum albumin (HSA), by using a heterobifunctional reagent, epsilon-maleimidocaproic acid N-hydrolrysuccinimide ester, to obtain a well-defined product. The sensitivity and the specificity of the peptide-specific ELISA were determined with a panel of 106 serum samples from individuals living in areas where visceral leishmaniasis is endemic ; sera from post-kala azar dermal leishmaniasis-infected patients and from individuals suffering from other infectious diseases were also included.