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Savoye, C ; Sabattier, R ; Charlier, M ; Spotheim-Maurizot, M

Sequence-modulated radiosensitization of DNA by copper ions

International Journal of Radiation Biology 70 (2) 189-198

par Administrateur - publié le , mis à jour le

Abstract :

Plasmid DNA and restriction fragments of 80 and 120 base pairs were irradiated with fast neutrons in the presence of CuCl2. The number of single and double strand breaks is higher in the presence than in the absence of Cu2+ ions. The radiosensitizing effect was lower for solutions of high compared with low ionic strength, and also lower for deoxygenated than for aerated solutions. This effect was inhibited by EDTA, catalase and Tris, but not by ethanol. Superoxide dismutase partially inhibited the effect of low copper concentrations (