Accueil > Publications > Recherche par années > Années 1990 > 1998

Gondeau, C ; Maurizot, JC ; Durand, M

Circular dichroism and UV melting studies on formation of an intramolecular triplex containing parallel T*A : T and G*G : C triplets : netropsin complexation with the triplex

Nucleic Acids Research 26 (21) 4996-5003

par Administrateur - publié le

Abstract :

We have used circular dichroism and UV absorption spectroscopy to characterize the formation and melting behaviour of an intramolecular DNA triple helix containing parallel T*A:T and G*G:C triplets. Our approach to induce and to stabilize a parallel tripler involves the oligonucleotide 5’-d(G(4)A(4)G(4)[T-4]C4T4C4- [T-4]G(4)T(4)G(4)) ([T-4] represents a stretch of four thymine residues). In a 10 mM sodium cacodylate, 0.2 mM disodium EDTA (pH 7) buffer, we have shown the following significant results.