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Arimondo, PB ; Barcelo, F ; Sun, JS ; Maurizot, JC ; Garestier, T ; Helene, C

Triple helix formation by (G,A)-containing oligonucleotides : Asymmetric sequence effect

Biochemistry 37 (47) 16627-16635

par Administrateur - publié le

Abstract :

Sequence effects on the stability of purine-motif (also called (G,A)-motif) triple helix have been investigated through mio symmetry-related systems : one of them had a 5’(GGA)(4)3’ core sequence of triplex-forming oligonucleotides (TFOs), whereas the other one had a reversed 5’(AGG)(4)3’ core sequence. These (G,A)-containing TFOs were prone to self-associate into intermolecular complexes at room temperature. The competition of TFOs’ self-association with triple helix formation was assessed, and minimized.