Accueil > Publications > Recherche par années > Années 1990 > 1999

Doan, BT ; Nezry, C ; Rene, L ; Badet, B ; Beloeil, JC

A heteronuclear and homonuclear filtering strategy for studying the structure of membrane peptides in non-deuterated phospholipid vesicles

Journal de Chimie Physique et de Physico-Chimie Biologique 95 (2) 221-224

par Administrateur - publié le

Abstract :

NMR study of membrane biomolecules comes up against a poor solubility in classical solvents. A strategy was elaborated to obtain structural information of peptides in non deuterated phospholipids vesicles. It is based on isotopic (HSQC-NOESY) and homonuclear selective filters, both using a fine water suppression. The method is illustrated with the substance P, a 11-residue membrane neuropeptide.