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Begusova, M ; Spotheim-Maurizot, M ; Michalik, V ; Charlier, M

Effect of ethidium bromide intercalation on DNA radiosensitivity

International Journal of Radiation Biology 76 (1) 1-9

par Administrateur - publié le

Abstract :

Purpose : To assess the influence of the intercalating drug ethidium bromide (EtBr) on the yields of single strand breaks (ssb) induced by fast neutrons in supercoiled pBR322 plasmid and in a linear DNA restriction fragment. Materials and methods : The yield of ssb in the plasmid was measured by agarose gel electrophoresis. The proportion of fragments bearing one ssb and the probability of breakage at each nucleotide site was determined using sequencing gel electrophoresis. The volume variations due to the intercalation of EtBr were calculated. The expected radio-modifying effect at each nucleotide site of the linear fragment was evaluated using a reported simulation procedure.