Accueil > Publications > Recherche par années > Années 2000 > 2002

Tahir, TA ; Berrin, JG ; Flatman, R ; Roussel, A ; Roepstorff, P ; Williamson, G ; Juge, N

Specific characterization of substrate and inhibitor binding sites of a glycosyl hydrolase family 11 xylanase from Aspergillus niger

Journal of Biological Chemistry 277 (46) 44035-44043

par Administrateur - publié le

Abstract :

The importance of aromatic and charged residues at the surface of the active site of a family 11 xylanase from Aspergillus niger was evaluated using site-directed mutagenesis. Ten mutant proteins were heterologously produced in Pichia pastoris, and their biochemical properties and kinetic parameters were determined. The specific activity of the Y6A, Y10A, Y89A, Y164A, and W172A mutant enzymes was drastically reduced.