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Lartia, R ; Asseline, U

New reagent for the preparation of oligonucleotides involving a 5 ’-thiophosphate or a 5 ’-phosphate group

Tetrahedron Letters 45 (31) 5949-5952

par Administrateur - publié le

Abstract :

We report here a novel, simple reagent enabling the chemical incorporation of a thiophospliate or a phosphate group at the 5’-end of oligonucleotides using very mild basic deprotection conditions. This method can be useful in the case of alkali sensitive modified oligonucleotides. This reagent also gives access to the preparation of bifunctional oligonucleotides with either a thiophosphate group at the 5’-end and a phosphate at the 3’-end, or two thiophosphate groups at both the 5’- and the 3’-ends, or a 5’-thiophosphate group and a 3’-amino-containing linker. (C) 2004 Elsevier Ltd. All rights reserved.