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Gras, J ; Sy, D ; Sverine, E ; Charlier, M ; Spotheim-Maurizot, M

Consequences of intramolecular dityrosine formation on a DNA-protein complex : a molecular modeling study

Radiation Physics and Chemistry 72 (2-3) 271-278

par Administrateur - publié le

Abstract :

Irradiation of the free lac repressor with gamma-rays abolishes protein’s ability to specifically bind operator DNA. A possible radiation-induced protein damage is a dityrosine (DTyr) formed by two spatially close radiation-induced tyrosyl radicals. We performed the molecular modeling of complexes between Operator DNA and DTyr-bearing parts (headpieces) of the repressor. The presence of DTyr affects the structure and the interactions between partners. A detailed analysis allows to conclude this damage can partially account for the loss of repressor ability to bind DNA. (C) 2004 Elsevier Ltd. All rights reserved.