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Fenyo, D ; Wang, QJ ; DeGrasse, JA ; Padovan, JC ; Cadene, M ; Chait BT

MALDI Sample Preparation : the Ultra Thin Layer Method (Videopublication)

Journal of Visualized Experiments, Cellular Biology Issue 3 (http://www.jove.com/index/details.stp?ID=192)

par Administrateur - publié le

Abstract :

This video demonstrates the preparation of an ultra-thin matrix/analyte layer for analyzing peptides and proteins by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS) [1, 2]. The ultra-thin layer method involves the production of a substrate layer of matrix crystals (alpha-cyano-4-hydroxycinnamic acid) on the sample plate, which serves as a seeding ground for subsequent crystallization of a matrix/analyte mixture. Advantages of the ultra-thin layer method over other sample deposition approaches (e.g. dried droplet) are that it provides (i) greater tolerance to impurities such as salts and detergents, (ii) better resolution, and (iii) higher spatial uniformity.