Grosse, S ; Aron, Y ; Thevenot, G ; Monsigny, M ; Fajac, I
Journal of Controlled Release 122 111-117
publié le , mis à jour le
We have studied the cytoskeletal involvement in the cellular trafficking of complexes made with plasmid/PEI or plasmid/lactosylated PEI in cystic fibrosis airway epithelial cells (Sigma CFTE29o-cells). Complexes were incubated in the presence of cytoskeletal inhibitors, and the number of transfected cells was determined by flow cytometry. Complexes were also generated with fluorescein-labeled PEI derivatives and the cell fluorescence intensity was determined by flow cytometry.