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Grosse, S ; Aron, Y ; Thevenot, G ; Monsigny, M ; Fajac, I

Cytoskeletal involvement in the cellular trafficking of plasmid/PEI derivative complexes

Journal of Controlled Release 122 111-117

par Administrateur - publié le , mis à jour le

Abstract :

We have studied the cytoskeletal involvement in the cellular trafficking of complexes made with plasmid/PEI or plasmid/lactosylated PEI in cystic fibrosis airway epithelial cells (Sigma CFTE29o-cells). Complexes were incubated in the presence of cytoskeletal inhibitors, and the number of transfected cells was determined by flow cytometry. Complexes were also generated with fluorescein-labeled PEI derivatives and the cell fluorescence intensity was determined by flow cytometry.