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Accueil > Publications > Recherche par années > Années 1990 > 1997

BernalMendez, E ; Boudvillain, M ; GonzalezVilchez, F ; Leng, M

Chemical versatility of transplatin monofunctional adducts within multiple site-specifically platinated DNA

Biochemistry 36 (24) 7281-7287

par Administrateur - publié le

Abstract :

The first step of the reaction between DNA and the antitumor drug cisplatin or its clinically inactive isomer transplatin yields monofunctional adducts, Most of the cisplatin monofunctional adducts further react and rather rapidly (t(1/2) smaller than a few hours) to form intrastrand and interstrand crosslinks. It is generally accepted that the clinical activity of cisplatin is related to the formation of bifunctional lesions, As concerns transplatin, several studies disagree on the rate of closure of the monofunctional adducts and the nature of the bifunctional lesions, In order to explain these discrepancies, we have prepared several duplexes containing a single monofunctional trans-[Pt(NH3)(2)(dG)Cl](+) adduct and zero to two monofunctional [Pt(dien)(dG)](2+) adducts at defined positions.