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Kieken, F., Loth, K., Van Nuland, N., Tompa, P. and Lenaerts, T.

Chemical shift assignments of the partially deuterated Fyn SH2–SH3 domain

Biomolecular NMR Assignments (2017)

par Frapart - publié le

Abstract :

Src Homology 2 and 3 (SH2 and SH3) are two key protein interaction modules involved in regulating the activity of many proteins such as tyrosine kinases and phosphatases by respective recognition of phosphotyrosine and proline-rich regions. In the Src family kinases, the inactive state of the protein is the direct result of the interaction of the SH2 and the SH3 domain with intra-molecular regions, leading to a closed structure incompetent with substrate modification. Here, we report the 1H, 15N and 13C backbone- and side-chain chemical shift assignments of the partially deuterated Fyn SH3-SH2 domain and structural differences between tandem and single domains.

Voir en ligne : 10.1007/s12104-017-9792-1