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Kieken F., Loth K., van Nuland N., Tompa P. and Lenaerts T.

Chemical shift assignments of the partially deuterated Fyn SH2-SH3 domain.

Biomolecular NMR assignments, (2018) 12, 117-122.

par Frapart - publié le

Abstract :

Src Homology 2 and 3 (SH2 and SH3) are two key protein interaction modules involved in regulating the activity of many proteins such as tyrosine kinases and phosphatases by respective recognition of phosphotyrosine and proline-rich regions. In the Src family kinases, the inactive state of the protein is the direct result of the interaction of the SH2 and the SH3 domain with intra-molecular regions, leading to a closed structure incompetent with substrate modification. Here, we report the (1)H, (15)N and (13)C backbone- and side-chain chemical shift assignments of the partially deuterated Fyn SH3-SH2 domain and structural differences between tandem and single domains.